Electroporation with nanosecond pulses and bleomycin or cisplatin results in efficient cell kill and low metal release from electrodes

• Nanopulses in combination with bleomycin or cisplatin successfully kill cells. are comparably effective as 8 × 100 ?s pulses electrochemotherapy. cause lower metal release from electrodes than pulses. Nanosecond electric have several potential advantages electroporation-based procedures over the conventional micro- and millisecond including low level of heating, reduced electrochemical reactions muscle contractions making them alluring for use biomedicine food industry. The aim this study was to evaluate if nanosecond can enhance cytotoxicity chemotherapeutics vitro quantify comparison commonly used effects pulse parameters (voltage, duration, number pulses) on cell membrane permeabilization, resealing survival after electroporation only electrochemotherapy were evaluated Chinese hamster ovary Application permeabilizing resulted successful kill. Higher extracellular concentrations – but not needed achieve same decrease eight pulses, however, tested still considerably compared doses clinical practice. Decreasing duration microseconds nanoseconds concomitantly increasing amplitude biological effect aluminum ions cuvettes.